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Identification and characterization of microsatellite markers for scleractinian coral Galaxea fascicularis and its symbiotic zooxanthellae

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Abstract

Nine polymorphic microsatellites markers were identified for Galaxea fascicularis symbionts and characterized in 29 samples from two populations. Four of them were derived from coral genome with 5–11 alleles, and the expected and observed heterozygosities ranging 0.67–0.89 and 0.25–0.69 respectively. Five were derived from zooxanthella genome amplified 0–3 alleles/locus per individual, while 2–4 alleles/locus with totally 13 genotypes in the two populations, which likely implied multiple DNA-polymorphic Symbiodinium species associated with single host and the populations. The genetic diversity of the two coral host populations was significantly greater than that of zooxanthella populations. These markers should be useful in various genetic applications involving both the coral host and the zooxanthella.

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Acknowledgments

This study was supported by the National Key Technology R&D Program (2009BAB44B03) and Hainan Natural Science Foundation (#311024).

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Authors and Affiliations

  1. Hainan Marine Development Plan and Design Research Institute, 15 Longkunbei Road, Haikou, 570125, Hainan, China

    Dandan Chen, Daoru Wang & Yuanchao Li

  2. Key Laboratory of Tropical Biological Resources of Ministry of Education, Ocean College, Marine Biology Laboratory Teaching Center, Hainan University, 58 Renmin Road, Haikou, 570228, Hainan, China

    Jintian Zhu, Xingxing Wu & Yan Wang

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  1. Dandan Chen
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  2. Daoru Wang
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  3. Jintian Zhu
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  4. Yuanchao Li
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  5. Xingxing Wu
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  6. Yan Wang
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Correspondence to Yan Wang.

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Chen, D., Wang, D., Zhu, J. et al. Identification and characterization of microsatellite markers for scleractinian coral Galaxea fascicularis and its symbiotic zooxanthellae. Conservation Genet Resour 5, 741–743 (2013). https://doi.org/10.1007/s12686-013-9895-7

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  • DOI: https://doi.org/10.1007/s12686-013-9895-7

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